in fusion cloning technique (TaKaRa)
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in fusion cloning technique
In Fusion Cloning Technique, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1541 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/in+fusion+hd+cloning+technique/In-Fusion+HD+Cloning+Plus/bio_rxiv__64898__2026__04__08__717226-182-13-16
Average 99 stars, based on 1541 article reviews
In Fusion Cloning Technique, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1541 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/in+fusion+hd+cloning+technique/In-Fusion+HD+Cloning+Plus/bio_rxiv__64898__2026__04__08__717226-182-13-16
Average 99 stars, based on 1541 article reviews
in fusion cloning technique - by Bioz Stars,
2026-09
99/100 stars
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Cloning:Article Title: Bacterial host and reporter gene optimization for genetically encoded whole cell biosensors. Article Snippet: .. Insertions of Pgapdh and Pars promoters were realized by the Article Title: Virulence of Agrobacterium tumefaciens requires lipid homeostasis mediated by the lysyl-phosphatidylglycerol hydrolase AcvB. Article Snippet: .. Therefore, base pairs 73 – 1368 of acvB (acvB gene devoid of its natural leader sequence) were PCR-amplified using oligonucleotides 1/2 and cloned into the NcoI/HindIII site of vector pET22b(+)Strep (Nicke et al., 2013) using the Article Title: Functional interaction between receptor tyrosine kinase MET and ETS transcription factors promotes prostate cancer progression Article Snippet: .. These complementary DNA fragments of ETV1 and ERG were then inserted into a retroviral plasmid vector plpcx puro using the Article Title: Functional interaction between receptor tyrosine kinase MET and ETS transcription factors promotes prostate cancer progression. Article Snippet: This method, developed by Eurofins Genomics, enables the specific genetic profiles of the cell lines to be detected accurately and reliably, guaranteeing their correct identification. .. These complementary DNA fragments of ETV1 and ERG were then inserted into a retroviral plasmid vector plpcx puro using the Article Title: Purification and Properties of Glycine Oxidase from Pseudomonas putida KT2440. Article Snippet: .. The amplified gene was inserted into the EcoRI site of the pCold I vector (Novagen, Madison, WI) by the Article Title: Virulence of Agrobacterium tumefaciens requires lipid homeostasis mediated by the lysyl-phosphatidylglycerol hydrolase AcvB. Article Snippet: .. A synthetic gene (Geneart) containing bases 1 – 72 of acvB followed by bases 100 – 1,416 from phoA (compare Table S1) was synthesized and cloned into the NcoI/HindIII sites of pTRC200 using the Article Title: Bacterial host and reporter gene optimization for genetically encoded whole cell biosensors. Article Snippet: .. 11 and 12), into pACYC184 plasmid (Rose 1988) at the EcoRI restriction site by Article Title: AcHKT1;2 is a candidate transporter mediating the influx of Na+ into the salt bladder of Atriplex canescens Article Snippet: Results Two AcHKT1 genes were cloned; AcHKT1;1 was largely expressed in roots, while AcHKT1;2 was mainly expressed in shoots and strongly induced by NaCl.. Heterologous expression of AcHKT1;2 aggravated the Na+-sensitive phenotype in yeast.. This result was further confirmed in Xenopus system, in which AcHKT1;2 exerted high selectivity for Na+, indicating that AcHKT1;2 functions as a plasma membrane-localized Na+ transporter and mediates robust Na+ influx at the cellular level. Sequencing:Article Title: Virulence of Agrobacterium tumefaciens requires lipid homeostasis mediated by the lysyl-phosphatidylglycerol hydrolase AcvB. Article Snippet: .. Therefore, base pairs 73 – 1368 of acvB (acvB gene devoid of its natural leader sequence) were PCR-amplified using oligonucleotides 1/2 and cloned into the NcoI/HindIII site of vector pET22b(+)Strep (Nicke et al., 2013) using the Polymerase Chain Reaction:Article Title: Virulence of Agrobacterium tumefaciens requires lipid homeostasis mediated by the lysyl-phosphatidylglycerol hydrolase AcvB. Article Snippet: .. Therefore, base pairs 73 – 1368 of acvB (acvB gene devoid of its natural leader sequence) were PCR-amplified using oligonucleotides 1/2 and cloned into the NcoI/HindIII site of vector pET22b(+)Strep (Nicke et al., 2013) using the Clone Assay:Article Title: Virulence of Agrobacterium tumefaciens requires lipid homeostasis mediated by the lysyl-phosphatidylglycerol hydrolase AcvB. Article Snippet: .. Therefore, base pairs 73 – 1368 of acvB (acvB gene devoid of its natural leader sequence) were PCR-amplified using oligonucleotides 1/2 and cloned into the NcoI/HindIII site of vector pET22b(+)Strep (Nicke et al., 2013) using the Article Title: Virulence of Agrobacterium tumefaciens requires lipid homeostasis mediated by the lysyl-phosphatidylglycerol hydrolase AcvB. Article Snippet: .. A synthetic gene (Geneart) containing bases 1 – 72 of acvB followed by bases 100 – 1,416 from phoA (compare Table S1) was synthesized and cloned into the NcoI/HindIII sites of pTRC200 using the Article Title: AcHKT1;2 is a candidate transporter mediating the influx of Na+ into the salt bladder of Atriplex canescens Article Snippet: Results Two AcHKT1 genes were cloned; AcHKT1;1 was largely expressed in roots, while AcHKT1;2 was mainly expressed in shoots and strongly induced by NaCl.. Heterologous expression of AcHKT1;2 aggravated the Na+-sensitive phenotype in yeast.. This result was further confirmed in Xenopus system, in which AcHKT1;2 exerted high selectivity for Na+, indicating that AcHKT1;2 functions as a plasma membrane-localized Na+ transporter and mediates robust Na+ influx at the cellular level. Plasmid Preparation:Article Title: Virulence of Agrobacterium tumefaciens requires lipid homeostasis mediated by the lysyl-phosphatidylglycerol hydrolase AcvB. Article Snippet: .. Therefore, base pairs 73 – 1368 of acvB (acvB gene devoid of its natural leader sequence) were PCR-amplified using oligonucleotides 1/2 and cloned into the NcoI/HindIII site of vector pET22b(+)Strep (Nicke et al., 2013) using the Article Title: Functional interaction between receptor tyrosine kinase MET and ETS transcription factors promotes prostate cancer progression Article Snippet: .. These complementary DNA fragments of ETV1 and ERG were then inserted into a retroviral plasmid vector plpcx puro using the Article Title: Functional interaction between receptor tyrosine kinase MET and ETS transcription factors promotes prostate cancer progression. Article Snippet: This method, developed by Eurofins Genomics, enables the specific genetic profiles of the cell lines to be detected accurately and reliably, guaranteeing their correct identification. .. These complementary DNA fragments of ETV1 and ERG were then inserted into a retroviral plasmid vector plpcx puro using the Article Title: Purification and Properties of Glycine Oxidase from Pseudomonas putida KT2440. Article Snippet: .. The amplified gene was inserted into the EcoRI site of the pCold I vector (Novagen, Madison, WI) by the Article Title: Bacterial host and reporter gene optimization for genetically encoded whole cell biosensors. Article Snippet: .. 11 and 12), into pACYC184 plasmid (Rose 1988) at the EcoRI restriction site by Article Title: AcHKT1;2 is a candidate transporter mediating the influx of Na+ into the salt bladder of Atriplex canescens Article Snippet: Results Two AcHKT1 genes were cloned; AcHKT1;1 was largely expressed in roots, while AcHKT1;2 was mainly expressed in shoots and strongly induced by NaCl.. Heterologous expression of AcHKT1;2 aggravated the Na+-sensitive phenotype in yeast.. This result was further confirmed in Xenopus system, in which AcHKT1;2 exerted high selectivity for Na+, indicating that AcHKT1;2 functions as a plasma membrane-localized Na+ transporter and mediates robust Na+ influx at the cellular level. Retroviral:Article Title: Functional interaction between receptor tyrosine kinase MET and ETS transcription factors promotes prostate cancer progression Article Snippet: .. These complementary DNA fragments of ETV1 and ERG were then inserted into a retroviral plasmid vector plpcx puro using the Article Title: Functional interaction between receptor tyrosine kinase MET and ETS transcription factors promotes prostate cancer progression. Article Snippet: This method, developed by Eurofins Genomics, enables the specific genetic profiles of the cell lines to be detected accurately and reliably, guaranteeing their correct identification. .. These complementary DNA fragments of ETV1 and ERG were then inserted into a retroviral plasmid vector plpcx puro using the Amplification:Article Title: Purification and Properties of Glycine Oxidase from Pseudomonas putida KT2440. Article Snippet: .. The amplified gene was inserted into the EcoRI site of the pCold I vector (Novagen, Madison, WI) by the Article Title: AcHKT1;2 is a candidate transporter mediating the influx of Na+ into the salt bladder of Atriplex canescens Article Snippet: Results Two AcHKT1 genes were cloned; AcHKT1;1 was largely expressed in roots, while AcHKT1;2 was mainly expressed in shoots and strongly induced by NaCl.. Heterologous expression of AcHKT1;2 aggravated the Na+-sensitive phenotype in yeast.. This result was further confirmed in Xenopus system, in which AcHKT1;2 exerted high selectivity for Na+, indicating that AcHKT1;2 functions as a plasma membrane-localized Na+ transporter and mediates robust Na+ influx at the cellular level. Transformation Assay:Article Title: Purification and Properties of Glycine Oxidase from Pseudomonas putida KT2440. Article Snippet: .. The amplified gene was inserted into the EcoRI site of the pCold I vector (Novagen, Madison, WI) by the Recombinant:Article Title: Purification and Properties of Glycine Oxidase from Pseudomonas putida KT2440. Article Snippet: .. The amplified gene was inserted into the EcoRI site of the pCold I vector (Novagen, Madison, WI) by the Synthesized:Article Title: Virulence of Agrobacterium tumefaciens requires lipid homeostasis mediated by the lysyl-phosphatidylglycerol hydrolase AcvB. Article Snippet: .. A synthetic gene (Geneart) containing bases 1 – 72 of acvB followed by bases 100 – 1,416 from phoA (compare Table S1) was synthesized and cloned into the NcoI/HindIII sites of pTRC200 using the Expressing:Article Title: AcHKT1;2 is a candidate transporter mediating the influx of Na+ into the salt bladder of Atriplex canescens Article Snippet: Results Two AcHKT1 genes were cloned; AcHKT1;1 was largely expressed in roots, while AcHKT1;2 was mainly expressed in shoots and strongly induced by NaCl.. Heterologous expression of AcHKT1;2 aggravated the Na+-sensitive phenotype in yeast.. This result was further confirmed in Xenopus system, in which AcHKT1;2 exerted high selectivity for Na+, indicating that AcHKT1;2 functions as a plasma membrane-localized Na+ transporter and mediates robust Na+ influx at the cellular level. |